probing 6,000 years of penguin poo for clues to the past
- Written by: Jamie Wood, Senior Lecturer in Ecology and Evolution, University of Adelaide
Studies of ancient DNA have tended to focus on frozen land in the northern hemisphere, where woolly mammoths and bison roamed. Meanwhile, Antarctica has received relatively little attention. We set out to change that.
The most suitable sediments are exposed near the coast of the icy continent, where penguins like to breed. Their poo is a rich source of DNA, providing information about the health of the population as well as what penguins have been eating.
Our new research opens a window on the past of Adélie penguins in Antarctica, going back 6,000 years. It also offers a surprise glimpse into the shrinking world of southern elephant seals over the past 1,000 years.
Understanding how these species coped with climate change in the past can help us prepare for the future. Wildlife in Antarctica faces multiple emerging threats and will likely need support to cope with the many challenges ahead.
A unique marine ecosystem
Adélie penguins are particularly sensitive to changes in their environment. This makes them what we call a “sentinel species”, providing an early warning of imbalance or dysfunction in the coastal ecosystem. Their poo also provides a record of how they responded to changes in the past.
In our new research, we excavated pits up to 80cm deep at ten Adélie penguin colonies along the 700km Ross Sea coastline. We then collected 156 sediment samples from different depths in each excavation.
Six of these colonies were still active, meaning birds return annually to breed. The other four had been abandoned at various times over the past 6,000 years.
From these sediments we generated 94 billion DNA sequences, which provided us with an unparalleled window into the past lives of Adélie penguins and their ecosystem.
We detected the DNA of several animal species besides Adélie penguins. These animals included two other birds, three seals and two soil invertebrates.
Not all of this DNA came from penguin poo. Our samples also contained DNA from feathers, hairs or skin cells of other species in the environment at the time.
Penguin population size and diet
When we took a closer look at the DNA from penguins of the present day, we found more genetic diversity in samples from larger colonies.
Recognising this relationship between genetic diversity and colony size enabled us to estimate the size of former colonies. We could also reconstruct population trends through time.
For example, in samples from active colonies, we found penguin genetic diversity increased as we sampled closer and closer to the surface. This may reflect population growth over the past century.
The DNA also revealed changes in penguin diets over time. Over the past 4,000 years, the penguins in the southern Ross Sea switched from mainly eating one type of fish – the bald notothen – to another, Antarctic silverfish.
The bald notothen lives beneath the sea ice, so this prey-switching was likely driven by a change in sea ice extent compared with the past.
Surprise! Elephant seals
We made an unexpected discovery at Cape Hallett, in the northern Ross Sea. This is the site of an active penguin colony.
Samples of sediment from close to the surface contained lots of penguin DNA and eggshell. But samples from further down, where penguin DNA and eggshell were scarce, contained DNA from southern elephant seals.
Today, elephant seals are uncommon visitors to the Antarctic continent, and breed on subantarctic islands including Macquarie, Campbell and Antipodes Islands. Yet, bones of elephant seal pups found along the Ross Sea coast indicate the species used to breed in the area.
Carbon dating of these bones indicate elephant seal colonies began disappearing from the southern Ross Sea around 1,000 years ago. Over the following 200 years, colonies in the northern Ross Sea began vanishing too.
As the climate cooled and the extent of sea ice increased, elephant seals could no longer access suitable breeding sites. These sites were then taken over by Adélie penguins who expanded into areas once occupied by seals.
Our DNA evidence suggests Cape Hallett was one of the last strongholds of southern elephant seals on the icy continent. But we may yet again see elephant seals breeding on the Antarctic mainland as the world warms and sea ice melts.
Reconstruction of the Ross Sea ecosystem, showing a variety of species identified using ancient environmental DNA from Adélie penguin colonies.
Artwork by Baijing / Centre for Evolutionary Organismal Biology at Zhejiang University
Even more ancient DNA in Antarctica
Our study spans the past 6,000 years, but our research suggests it would be possible to go even further back.
The DNA fragments we found were very well preserved, showing little of the damage expected in warmer climates.
So it should be possible to obtain much older DNA from sediments on land in Antarctica – maybe even 1 million-year-old DNA, as recently reported from Antarctic sediments beneath the ocean floor.
Worthy of lasting protection
In December 2017, 2.09 million square kilometres of the Ross Sea and adjoining Southern Ocean became the world’s largest marine protected area. Establishing the protection was a major achievement, yet it was only afforded for 35 years.
After 2052, continuation of the region’s protected status will require international agreement. Knowledge of the vulnerability of local species and their risk in the face of change will play a key role in informing the decision. Our research provides a case study for how ancient environmental DNA can contribute towards this understanding.
Authors: Jamie Wood, Senior Lecturer in Ecology and Evolution, University of Adelaide
